APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human ENaC Alpha.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
sodium channel epithelial 1 alpha subunit , BESC2 , ENaCa , ENaCalpha , LIDLS3 , SCNEA , SCNN1
Cellular Localization
Apical cell membrane,Cell projection, cilium,Cytoplasm,Cell projection, cilium, flagellum
Background
Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2009]
Database
Research Area
DATA IMAGES
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GTX640465 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ENaC Alpha (GTX640465) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX640465 IHC-P Image
ENaC Alpha antibody [HL3027] detects ENaC Alpha protein by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.ENaC Alpha stained by ENaC Alpha antibody [HL3027] (GTX640465) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX640465 IHC-P Image
ENaC Alpha antibody [HL3027] detects ENaC Alpha protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.ENaC Alpha stained by ENaC Alpha antibody [HL3027] (GTX640465) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX640465 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ENaC Alpha antibody [HL3027] (GTX640465) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
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REVIEW
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